6''-O-Acetylglycitin

We used twelve isoflavone standard compounds, including six aglycones (DE, GE, and GLE) and glycosides (DG, GEG, and GLG) that were purchased from Weikeqi Bio-Technology Co., Ltd. (China); the other six acetyl glycosides (AD, AG, and AGL) and malonyl glycosides (MD, MG, and MGL) were purchased from Wako Pure Chemical Industries Co., Ltd. (Japan).  |||Identification and quantification of twelve isoflavones (DG, GLG, GEG, DE, GLE, GE, AD, AGL, AG, MD, MGL, and MG) and eight anthocyanins (DEA, DEL, CYA, CYL, PET, PEL, PEO, and CYC) were performed by external standardization using an Agilent 1260-series HPLC system equipped with a mass spectrometric detector (Agilent Quadrupole LC/MS 6120). Identification of the above isoflavones and anthocyanins was achieved by comparing their retention times in the HPLC chromatogram and the mass spectral information with those of the standard compounds.